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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
23/09/2019 |
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Actualizado : |
23/09/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 1 |
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Autor : |
COUTO, D.; STRANSFELD, L.; ARRUABARRENA, A.; ZIPFEL, C.; LOZANO-DURÁN, R. |
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Afiliación : |
DANIEL COUTO, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; LENA STRANSFELD, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CYRIL ZIPFEL, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ROSA LOZANO-DURÁN, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; Shanghai Institutes of Biological Sciences, Shanghai Center for Plant Stress Biology (PSC), Chinese Academy of Sciences, China. |
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Título : |
Broad application of a simple and affordable protocol for isolating plant RNA. (Short Report). |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
BMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS. |
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ISSN : |
1756-0500 |
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DOI : |
10.1186/s13104-015-1119-7 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published on: 16 April 2015. |
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Contenido : |
Abstract View references (4)
Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide.
© 2015 Couto et al.; licensee BioMed Central. |
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Palabras claves : |
Affordable; Arabidopsis; Protocol; RNA; Tomato; Wheat. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13321/1/Couto-et-al.-2015.-BMC-Research-Notes.pdf
https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-015-1119-7
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Marc : |
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100 1 $aCOUTO, D.
245 $aBroad application of a simple and affordable protocol for isolating plant RNA. (Short Report).$h[electronic resource]
260 $c2015
500 $aArticle history: Published on: 16 April 2015.
520 $aAbstract View references (4) Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide. © 2015 Couto et al.; licensee BioMed Central.
653 $aAffordable
653 $aArabidopsis
653 $aProtocol
653 $aRNA
653 $aTomato
653 $aWheat
700 1 $aSTRANSFELD, L.
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700 1 $aZIPFEL, C.
700 1 $aLOZANO-DURÁN, R.
773 $tBMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS.
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